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Title: Food as a source for quorum sensing inhibitors : Iberin from horseradish revealed as a quorum sensing inhibitor of Pseudomonas aeruginosa
Authors: Givskov, Michael
Jakobsen, Tim Holm
Bragason, Steinn Kristinn
Phipps, Richard Kerry
Christensen, Louise Dahl
Gennip, Maria van
Alhede, Morten
Skindersoe, Mette Eline
Larsen, Thomas Ostenfeld
Høiby, Niels
Bjarnsholt, Thomas
Issue Date: 2012
Source: Jakobsen, T. H., Bragason, S. K., Phipps, R. K., Christensen, L. D., Gennip, M. v., Alhede, M., et al. (2012). Food as a source for quorum sensing inhibitors: Iberin from horseradish revealed as a quorum sensing inhibitor of Pseudomonas aeruginosa. Applied and Environmental Microbiology, 78(7), 2410-2421.
Series/Report no.: Applied and environmental microbiology
Abstract: Foods with health-promoting effects beyond nutritional values have been gaining increasing research focus in recent years, although not much has been published on this subject in relation to bacterial infections. With respect to treatment, a novel antimicrobial strategy, which is expected to transcend problems with selective pressures for antibiotic resistance, is to interrupt bacterial communication, also known as quorum sensing (QS), by means of signal antagonists, the so-called QS inhibitors (QSIs). Furthermore, QSI agents offer a potential solution to the deficiencies associated with use of traditional antibiotics to treat infections caused by bacterial biofilms and multidrug-resistant bacteria. Several QSIs of natural origin have been identified, and in this study, several common food products and plants were extracted and screened for QSI activity in an attempt to isolate and characterize previously unknown QSI compounds active against the common opportunistic pathogen Pseudomonas aeruginosa. Several extracts displayed activity, but horseradish exhibited the highest activity. Chromatographic separation led to the isolation of a potent QSI compound that was identified by liquid chromatography-diode array detector-mass spectrometry (LC-DAD-MS) and nuclear magnetic resonance (NMR) spectroscopy as iberin—an isothiocyanate produced by many members of the Brassicaceae family. Real-time PCR (RT-PCR) and DNA microarray studies showed that iberin specifically blocks expression of QS-regulated genes in P. aeruginosa.
ISSN: 0099-2240
DOI: 10.1128/AEM.05992-11
Rights: © 2012 American Society for Microbiology
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCELSE Journal Articles

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