Predefinable colorimetric quantum-dot barcodes with simple and express identification algorithm
Gong Haiqing, Thomas
Date of Issue2013
School of Mechanical and Aerospace Engineering
Specific fluorescent profiles were created by loading quantum-dot (Qdot) mixtures in liquid cores of monodispersed polymer microcapsules, which were used as colorimetric barcodes for small object identification. Since the emission intensities of Qdot-loaded liquid cores maintain a linear relation to the Qdot concentrations and the Qdots in the liquid cores with different emission peaks have no obvious interference, the colorimetric barcodes can be predefined by the compositions of Qdot mixtures. The colorimetric barcodes can be identified easily by recording the emission intensities of the encoded microcapsules at respective Qdot emission peaks with a simple and express algorithm, which are suitable to conduct high-throughput multiplexed assays by flow cytometer for biological screening applications.
© 2013 Optical Society of America. This paper was published in Applied Optics and is made available as an electronic reprint (preprint) with permission of Optical Society of America. The paper can be found at the following official DOI: [http://dx.doi.org/10.1364/AO.52.000866]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.