Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
Ng, Ka Ming
Luo, Kathy Qian
Tang, Ben Zhong
Date of Issue2012
School of Chemical and Biomedical Engineering
A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.
© 2012 The Royal Society of Chemistry.