dc.contributor.authorNarayanan, Ranjani
dc.contributor.authorZhu, Li
dc.contributor.authorVelmurugu, Yogambigai
dc.contributor.authorRoca, Jorjethe
dc.contributor.authorKuznetsov, Serguei V.
dc.contributor.authorPrehna, Gerd
dc.contributor.authorLapidus, Lisa J.
dc.contributor.authorAnsari, Anjum
dc.date.accessioned2013-07-12T02:16:29Z
dc.date.available2013-07-12T02:16:29Z
dc.date.copyright2012en_US
dc.date.issued2012
dc.identifier.citationNarayanan, R., Zhu, L., Velmurugu, Y., Roca, J., Kuznetsov, S. V., Prehna, G., et al. (2012). Exploring the Energy Landscape of Nucleic Acid Hairpins Using Laser Temperature-Jump and Microfluidic Mixing. Journal of the American Chemical Society, 134(46), 18952-18963.en_US
dc.identifier.urihttp://hdl.handle.net/10220/11261
dc.description.abstractWe have investigated the multidimensionality of the free energy landscape accessible to a nucleic acid hairpin by measuring the relaxation kinetics in response to two very different perturbations of the folding/unfolding equilibrium, either a laser temperature-jump or ion-jump (from rapid mixing with counterions). The two sets of measurements carried out on DNA hairpins (4 or 5 base pairs in the stem and 21-nucleotide polythymine loop), using FRET between end labels or fluorescence of 2-aminopurine in the stem as conformational probes, yield distinctly different relaxation kinetics in the temperature range 10–30 °C and salt range 100–500 mM NaCl, with rapid mixing exhibiting slower relaxation kinetics after an initial collapse of the chain within 8 μs of the counterion mixing time. The discrepancy in the relaxation times increases with increasing temperatures, with rapid mixing times nearly 10-fold slower than T-jump times at 30 °C. These results rule out a simple two-state scenario with the folded and unfolded ensemble separated by a significant free energy barrier, even at temperatures close to the thermal melting temperature Tm. Instead, our results point to the scenario in which the conformational ensemble accessed after counterion condensation and collapse of the chain is distinctly different from the unfolded ensemble accessed with T-jump perturbation. Our data suggest that, even at temperatures in the vicinity of Tm or higher, the relaxation kinetics obtained from the ion-jump measurements are dominated by the escape from the collapsed state accessed after counterion condensation.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of the American chemical societyen_US
dc.rights© 2012 American Chemical Societyen_US
dc.titleExploring the energy landscape of nucleic acid hairpins using laser temperature-jump and microfluidic mixingen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1021/ja301218e


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record