Effects of F’, F-ANA and LNA modification on the stability of DNA G-quadruplex

Abstract

Quadruplex is formed by G-tetrads (Fig. a) stacking and it forms at Guanine-rich region. G-quadruplex is reported to have anti-cancer and anti-HIV effect [1]. T0 quadruplex (Fig. b) studied in this project is in (4+0) conformation and has very high symmetry [2]. F’, F-ANA and LNA (Fig. d-f) are modified sugars and they are highly stable in biological condition, easy to synthesize and soluble in water. Thanks to these beneficial properties, F’, F-ANA and LNA are promising candidates for DNA and RNA based drug design [3-5]. In this project, The effects of F’, F-ANA and LNA modifications on thermal stability of G-quadruplex were studied on the platform of T0 G-quadruplex and characterized by UV melting. [1st Award]