Investigation of cell morphology by the TRUImagE digital holographic microscopy system
Chee, Oi Choo
Chai, Kim Kheong
Asundi, Anand Krishna
Date of Issue2012
Biophotonics: Photonic Solutions for Better Health Care (3rd : 2012 : Brussels, Belgium)
School of Mechanical and Aerospace Engineering
We report the development of the Three-dimensional Real-time Uninvasive Imaging and Evaluation (TRUImagE) system based on digital holographic microscopy to study the morphological changes in cells undergoing photodynamic therapyinduced cell death. The optical system, based on the Michelson interferometer and configured in transmission mode, and the sample holder incorporating a stage incubator have been developed for monitoring various tumorigenic cell samples without the use of markers. Off-axis digital holograms were recorded with a CCD sensor and numerically reconstructed to provide quantitative phase imaging and 3D morphology of the cells in real time. The system was used to continuously monitor and study, at different time points, the changes in cells after incubation with the photosensitizer followed by activation by the appropriate light dose. Results obtained from the TRUImagE system and biochemical assays will be given.
© 2012 SPIE. This paper was published in Proceedings of SPIE - Biophotonics: Photonic Solutions for Better Health Care III and is made available as an electronic reprint (preprint) with permission of SPIE. The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/12.921613]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.