Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/97943
Title: Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion
Authors: Ravindran, Anita
Sze, Siu Kwan
Adav, Sunil S.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Ravindran, A., Adav, S. S., & Sze, S. K. (2012). Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion. Process Biochemistry, 47(12), 2440-2448.
Series/Report no.: Process biochemistry
Abstract: Biorefinery of renewable lignocellulosic biomass to biochemical and biofuel is a promising technology to mitigate global warming and fuel shortage but hydrolysis of recalcitrant lignocellulose to its constitutive components is the bottleneck of the process. This work isolated and characterized a new lignocellulose degrading filamentous fungus from decomposing wood in mangrove area. The strain was identified as Coniochaeta sp. according to ITS rRNA sequences and its phylogenic analysis. The extracellular lignocellulolytic enzymes of this fungal strain, when grown on corn stover, were profiled by LC–MS/MS and exponentially modified protein abundance index (emPAI) based label-free quantitative proteomics approach. We identified 107 potential lignocellulolytic enzymes and their functional classification revealed unique extracellular enzyme system constituting multienzyme complexes of cellulases (29%), hemicellulases (17%), glycoside hydrolases (10%), proteases and peptidases (24%), lignin degrading enzymes (7%) and hypothetical proteins (13%). The growth behavior, biochemical assay and LC–MS/MS analysis of secretome by isolated fungal strain revealed its lignocellulose degradation potential when cultivated with corn stover as a major carbon source.
URI: https://hdl.handle.net/10356/97943
http://hdl.handle.net/10220/12318
ISSN: 1359-5113
DOI: http://dx.doi.org/10.1016/j.procbio.2012.10.003
Rights: © 2012 Elsevier Ltd.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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