Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/97900
Title: Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA
Authors: Nordin, N.
Engman, H.
Lundback, A.-K.
Phua, T.
Jong, Agnes Jin Oi
Cornvik, Tobias Carl
Guskov, Albert
Reynaud, Aline
Eshaghi, Said
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Guskov, A., Nordin, N., Reynaud, A., Engman, H., Lundback, A. K., Jong, A. J. O., et al. (2012). Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA. Proceedings of the national academy of sciences, 109(45), 18459-18464.
Series/Report no.: Proceedings of the national academy of sciences
Abstract: Despite the importance of Mg2+ for numerous cellular activities, the mechanisms underlying its import and homeostasis are poorly understood. The CorA family is ubiquitous and is primarily responsible for Mg2+ transport. However, the key questions—such as, the ion selectivity, the transport pathway, and the gating mechanism—have remained unanswered for this protein family. We present a 3.2 Å resolution structure of the archaeal CorA from Methanocaldococcus jannaschii, which is a unique complete structure of a CorA protein and reveals the organization of the selectivity filter, which is composed of the signature motif of this family. The structure reveals that polar residues facing the channel coordinate a partially hydrated Mg2+ during the transport. Based on these findings, we propose a unique gating mechanism involving a helical turn upon the binding of Mg2+ to the regulatory intracellular binding sites, and thus converting a polar ion passage into a narrow hydrophobic pore. Because the amino acids involved in the uptake, transport, and gating are all conserved within the entire CorA family, we believe this mechanism is general for the whole family including the eukaryotic homologs.
URI: https://hdl.handle.net/10356/97900
http://hdl.handle.net/10220/12345
DOI: http://dx.doi.org/10.1073/pnas.1210076109
Rights: © 2012 National Academy of Sciences. This paper was published in Proceedings of the national academy of sciences and is made available as an electronic reprint (preprint) with permission of National Academy of Sciences. The paper can be found at the following official DOI: [http://dx.doi.org/10.1073/pnas.1210076109]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
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