Following G-quadruplex formation by its intrinsic fluorescence
Dao, Nguyen Thuan
Phan, Anh Tuân
Date of Issue2011
School of Physical and Mathematical Sciences
We characterized and compared the fluorescence properties of various well-defined G-quadruplex structures. The increase of intrinsic fluorescence of G-rich DNA sequences when they form G-quadruplexes can be used to monitor the folding and unfolding of G-quadruplexes as a function of cations and temperature. The temperature-dependent fluorescence spectra of different G-quadruplexes also exhibit characteristic patterns. Thus, the stability and possibly also the structure of G-quadruplexes can be characterized and distinguished by their intrinsic fluorescence spectra.
© 2011 Federation of European Biochemical Societies. This is the author created version of a work that has been peer reviewed and accepted for publication in FEBS letter, published by Elsevier B.V. on behalf of Federation of European Biochemical Societies. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1016/j.febslet.2011.11.004].