Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/99013
Title: Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor
Authors: Nguyen, Binh Thi Thanh
Peh, Alister En Kai
Chee, Celine Yue Ling
Fink, Katja
Chow, Vincent T. K.
Ng, Mary M. L.
Toh, Chee-Seng
Issue Date: 2012
Source: Nguyen, B. T. T., Peh, A. E. K., Chee, C. Y. L., Fink, K., Chow, V. T., Ng, M. M.,& Toh, C. S. (2012). Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor. Bioelectrochemistry, 88, 15-21.
Series/Report no.: Bioelectrochemistry
Abstract: The Faradaic electrochemical impedance technique is employed to characterize the impedance change of a nanoporous alumina biosensor in response towards the specific binding of dengue serotype 2 (Denv2) viral particles to its serotype 2-specific immunoglobulin G antibody within the thin alumina layer. The optimal equivalent circuit model that matches the impedimetric responses of the sensor describes three distinct regions: the electrolyte solution (Rs), the porous alumina channels (including biomaterials) (Q1, R1) and the conductive electrode substrate layer (Q2, R2). Both channel resistance R1 and capacitance Q1 change in response to the increase of the Denv2 virus concentration. A linear relationship between R1 and Denv2 concentration from 1 to 900 plaque forming unit per mL (pfu mL− 1) can be derived using Langmuir–Freundlich isotherm model. At 1 pfu mL− 1 Denv2 concentration, R1 can be distinguished from that of the cell culture control sample. Moreover, Q1 doubles when Denv2 is added but remains unchanged in the presence of two other non-specific viruses — West Nile virus and Chikungunya virus indicates biosensor specificity can be quantitatively measured using channel capacitance.
URI: https://hdl.handle.net/10356/99013
http://hdl.handle.net/10220/12707
ISSN: 1567-5394
DOI: http://dx.doi.org/10.1016/j.bioelechem.2012.04.006
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SPMS Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.