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|Title:||Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery||Authors:||Yan, Jiang
Eom, Khee Dong
Tam, James P.
|Keywords:||DRNTU::Science::Biological sciences||Issue Date:||2011||Source:||Yan, J., Korolev, N., Eom, K. D., Tam, J. P.,& Nordenskiöld, L. (2012). Biophysical Properties and Supramolecular Structure of Self-Assembled Liposome/ε-Peptide/DNA Nanoparticles: Correlation with Gene Delivery. Biomacromolecules, 13(1), 124-131.||Series/Report no.:||Biomacromolecules||Abstract:||Using solid-phase synthesis, lysine can be oligomerized by a reaction of the peptide carboxylate with the ε-amino group to produce nontoxic, biodegradable cationic peptides, ε-oligo(l-lysines). Here α-substituted derivatives of such ε-oligo(l-lysines) containing arginine and histidine in the side chain were tested as vectors for in vitro gene delivery. Combination of ε-oligolysines with the cationic lipid DOTAP and plasmid DNA resulted in transfection efficiency exceeding that of DOTAP alone, without significant increase in cytotoxicity. Synchrotron small-angle X-ray scattering studies revealed self-assembly of the DOTAP, ε-oligolysines, and DNA to ordered lamellar complexes. High transfection efficiency of the nanoparticles correlates with increase in zeta potential above +20 mV and requires particle size to be below 500 nm. The synergistic effect of branched ε-oligolysines and DOTAP in gene delivery can be explained by the increase in surface charge and by the supramolecular structure of the DOTAP/ε-oligolysine/DNA nanoparticles.||URI:||https://hdl.handle.net/10356/99003
|Appears in Collections:||SBS Journal Articles|
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