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https://hdl.handle.net/10356/98838
Title: | Isolating a trimer intermediate in the self-assembly of E2 protein cage | Authors: | Peng, Tao Lee, Hwankyu Lim, Sierin |
Issue Date: | 2012 | Source: | Peng, T., Lee, H.,& Lim, S. (2012). Isolating a Trimer Intermediate in the Self-Assembly of E2 Protein Cage. Biomacromolecules, 13(3), 699-705. | Series/Report no.: | Biomacromolecules | Abstract: | Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus, with a virus-like caged structure, has drawn much attention for its potential application as a nanocapsule. To investigate its self-assembly process from subunits to a spherical protein cage, we truncate the C-terminus of the E2 subunit. The redesigned protein subunit shows dynamic transition between monomer and trimer, but not the integrate 60-mer. The results indicate the role of the trimer as the intermediate and building block during the self-assembly of the E2 protein cage. In combination with the molecular dynamics simulations results, we conclude that the C-terminus modulates the self-assembly of the E2 protein cage from trimer to 60-mer. This investigation elucidates the role of the intersubunit interactions in engineering other functionalities in other caged structure proteins. | URI: | https://hdl.handle.net/10356/98838 http://hdl.handle.net/10220/12755 |
DOI: | 10.1021/bm201587q | Schools: | School of Chemical and Biomedical Engineering | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
Appears in Collections: | SCBE Journal Articles |
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