Axicon lens-based cone shell configuration for depth-sensitive fluorescence measurements in turbid media
Ong, Yi Hong
Date of Issue2013
School of Chemical and Biomedical Engineering
We have developed a novel noncontact setup to implement a cone shell illumination and detection configuration using axicon lenses for depth-sensitive fluorescence measurements. The setup was demonstrated experimentally to be capable of detecting fluorescence from a two-layered turbid agar phantom with a larger sensitivity to the deep layer and a larger range of sensitivity to either layer than a conventional cone configuration implemented by a microscope objective lens. Furthermore, the axicon lens-based setup eliminates the need of moving the objective lens up or down to achieve depth-sensitive measurements, which effectively improves the consistency of optical coupling and thus would be preferred in a clinical setting.
© 2013 Optical Society of America. This paper was published in Optics Letters and is made available as an electronic reprint (preprint) with permission of Optical Society of America. The paper can be found at the following official DOI: [http://dx.doi.org/10.1364/OL.38.002647]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.