Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/98069
Title: A lower limit of detection for atrazine was obtained using bioluminescent reporter bacteria via a lower incubation temperature
Authors: Marks, Robert S.
Ionescu, Rodica E.
Jia, Kun
Eltzov, Evgeni
Toury, Timothée
Issue Date: 2012
Source: Jia, K., Eltzov, E., Toury, T., Marks, R. S.,& Ionescu, R. E. (2012). A lower limit of detection for atrazine was obtained using bioluminescent reporter bacteria via a lower incubation temperature. Ecotoxicology and Environmental Safety, 84, 221-226.
Series/Report no.: Ecotoxicology and environmental safety
Abstract: The present article reports on the influence of various atrazine concentrations to the response of genetically modified Escherichia coli TV1061 bacterial cells while modulating the experimental conditions. Interesting increases of bioluminescence signals are recorded for E. coli TV1061 bacteria in the presence of 10 μg/mL atrazine concentration named “high-toxicity bacteria alert” when compared with 1 μg/mL –10 fg/mL atrazine termed “low-toxicity bacteria alert”. Detecting the effect of atrazine via its effect on bioluminescence of bacteria has been carried out by two consecutive measurements (fresh and overnight modes) at different concentrations of analyte. We have shown that a more precise discrimination at lower-toxicity concentrations can be obtained through overnight incubation of bacteria with the analyte at 4 °C. In addition, centrifugation of bacterial cells and analyte dilutions has been performed in order to ensure a better interaction between the insoluble atrazine pesticide and the bacterial cells.
URI: https://hdl.handle.net/10356/98069
http://hdl.handle.net/10220/13252
ISSN: 0147-6513
DOI: http://dx.doi.org/10.1016/j.ecoenv.2012.07.009
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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