Oxidation of DNA bases influenced by the presence of other bases
Author
Shuhua Goh, Madeline
Pumera, Martin
Date of Issue
2012School
School of Physical and Mathematical Sciences
Abstract
Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of
one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak
width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced
graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more
significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses
were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly
larger when only one or two other bases were present. Our findings are of paramount importance for future developments
in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical
biosensing systems.
Type
Journal Article
Series/Journal Title
Electroanalysis
Collections
http://dx.doi.org/10.1002/elan.201200036
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