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Title: New insights into the brain protein metabolism of Gastrodia elata-treated rats by quantitative proteomics
Authors: Manavalan, Arulmani
Feng, Lin
Sze, Siu Kwan
Hu, Jiang-Miao
Heese, Klaus
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Manavalan, A., Feng, L., Sze, S. K., Hu, J., & Heese, K. (2012). New insights into the brain protein metabolism of Gastrodia elata-treated rats by quantitative proteomics. Journal of proteomics, 75(8), 2468–2479.
Series/Report no.: Journal of proteomics
Abstract: Gastrodia elata (tianma) is a traditional Chinese herbal medicine (TCM) often used for the treatment of cerebrovascular diseases. In this study, we investigated the effects of tianma on the brain protein metabolism by quantitative proteomics to gain evidence for a direct relationship between tianma treatment and brain functions. One-year-old rats were treated with tianma (~ 2.5 g/kg/day) for 3 months and the brain tissue proteome was analyzed by using the iTRAQ (isobaric tag for relative and absolute quantification) technology. According to our results, the long-term treatment with tianma could modulate the brain protein metabolism at the proteome level by down-regulating the expressions of various proteins, such as Gnao1 and Dctn2, which are related to neuronal growth cone control and synaptic activities. In addition, tianma treatment also induced the up-regulation of molecular chaperons and proteins related to the misfolded protein response, like Anxa5, and also other proteins involved in Huntington's disease (HD) (e.g. Pacsin1 and Arf3). Concluding, tianma could eventually contribute to activities related to synaptic plasticity and neuro-restorative processes and thus might be a novel candidate agent for the treatment of neurodegenerative diseases by regulating the brain proteome.
DOI: 10.1016/j.jprot.2012.02.029
Fulltext Permission: none
Fulltext Availability: No Fulltext
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