Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103661
Title: A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation
Authors: Tan, Lay Poh
Yu, Ting
Chua, Chee Kai
Tay, Chor Yong
Wen, Feng
Yu, Haiyang
Chan, Jerry Kok Yen
Chong, Mark Seow Khoon
Leong, David Tai Wei
Keywords: DRNTU::Engineering::Chemical engineering::Biochemical engineering
Issue Date: 2013
Source: Yu, T., Chua, C. K., Tay, C. Y., Wen, F., Yu, H., Chan, J. K. Y., et al. (2013). A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation. Macromolecular bioscience, 13(6), 799-807.
Series/Report no.: Macromolecular bioscience
Abstract: Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone-marrow-derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well-aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment.
URI: https://hdl.handle.net/10356/103661
http://hdl.handle.net/10220/16726
ISSN: 1616-5187
DOI: http://dx.doi.org/10.1002/mabi.201200481
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MAE Journal Articles
MSE Journal Articles
SCBE Journal Articles

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