Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/102469
Title: A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay
Authors: Zhang, Rui
Gong Haiqing, Thomas
Zeng, Xu Dong
Sze, Chun Chau
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2013
Source: Zhang, R., Gong, H. T., Zeng, X. D., & Sze, C. C. (2013). A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay. Analytical and bioanalytical chemistry, 405(12), 4277-4282.
Series/Report no.: Analytical and bioanalytical chemistry
Abstract: A high-throughput microfluidic poly-(dimethylsiloxane) biochip was developed to quantify bacterial adhesion to single host cells by real-time PCR assay. The biochip is simply structured with a two-dimensional array of 900 micro-wells, one inlet, and one outlet micro-channels. Isolation of single infected host cells into the individual micro-wells of the biochip was achieved by one-step vacuum-driven microfluidics. The adhered bacterial cells were then quantified by direct on-chip real-time PCR assay with single-bacterium-detection sensitivity. The performance of this microfluidic platform was demonstrated through profiling of the association of a common bacterial pathogen, Pseudomonas aeruginosa, to single host human lung epithelial A549 cells, revealing an adherence distribution that has not been previously reported. This microfluidic platform offers a simple and effective tool for biologists to analyze pathogen–host interaction at the single-cell level without the necessities of fluorescence labeling. The chip can similarly be used for other PCR-based applications requiring single-cell analysis.
URI: https://hdl.handle.net/10356/102469
http://hdl.handle.net/10220/16867
ISSN: 1618-2642
DOI: 10.1007/s00216-013-6826-0
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MAE Journal Articles

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