dc.contributor.authorZhang, Rui
dc.contributor.authorGong Haiqing, Thomas
dc.contributor.authorZeng, Xu Dong
dc.contributor.authorSze, Chun Chau
dc.date.accessioned2013-10-25T01:36:55Z
dc.date.available2013-10-25T01:36:55Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationZhang, R., Gong, H. T., Zeng, X. D., & Sze, C. C. (2013). A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay. Analytical and bioanalytical chemistry, 405(12), 4277-4282.en_US
dc.identifier.issn1618-2642en_US
dc.identifier.urihttp://hdl.handle.net/10220/16867
dc.description.abstractA high-throughput microfluidic poly-(dimethylsiloxane) biochip was developed to quantify bacterial adhesion to single host cells by real-time PCR assay. The biochip is simply structured with a two-dimensional array of 900 micro-wells, one inlet, and one outlet micro-channels. Isolation of single infected host cells into the individual micro-wells of the biochip was achieved by one-step vacuum-driven microfluidics. The adhered bacterial cells were then quantified by direct on-chip real-time PCR assay with single-bacterium-detection sensitivity. The performance of this microfluidic platform was demonstrated through profiling of the association of a common bacterial pathogen, Pseudomonas aeruginosa, to single host human lung epithelial A549 cells, revealing an adherence distribution that has not been previously reported. This microfluidic platform offers a simple and effective tool for biologists to analyze pathogen–host interaction at the single-cell level without the necessities of fluorescence labeling. The chip can similarly be used for other PCR-based applications requiring single-cell analysis.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesAnalytical and bioanalytical chemistryen_US
dc.subjectDRNTU::Science::Biological sciences
dc.titleA high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assayen_US
dc.typeJournal Article
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00216-013-6826-0


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