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|Title:||Nanoparticle based galectin-1 gene silencing, implications in methamphetamine regulation of HIV-1 infection in monocyte derived macrophages||Authors:||Nair, Bindukumar B.
Reynolds, Jessica L.
Mahajan, Supriya D.
Sykes, Donald E.
Prasad, Paras N.
Schwartz, Stanley A.
|Keywords:||DRNTU::Engineering::Electrical and electronic engineering||Issue Date:||2012||Source:||Reynolds, J. L., Law, W.-C., Mahajan, S. D., Aalinkeel, R., Nair, B., Sykes, D. E., et al. (2012). Nanoparticle based galectin-1 gene silencing, implications in methamphetamine regulation of HIV-1 infection in monocyte derived macrophages. Journal of neuroimmune pharmacology, 7(3), 673-685.||Series/Report no.:||Journal of neuroimmune pharmacology||Abstract:||Galectin-1, an adhesion molecule, is expressed in macrophages and implicated in human immunodeficiency virus (HIV-1) viral adsorption. In this study, we investigated the effects of methamphetamine on galectin-1 production in human monocyte derived macrophages (MDM) and the role of galectin-1 in methamphetamine potentiation of HIV-1 infection. Herein we show that levels of galectin-1 gene and protein expression are significantly increased by methamphetamine. Furthermore, concomitant incubation of MDM with galectin-1 and methamphetamine facilitates HIV-1 infection compared to galectin-1 alone or methamphetamine alone. We utilized a nanotechnology approach that uses gold nanorod (GNR)-galectin-1 siRNA complexes (nanoplexes) to inhibit gene expression for galectin-1. Nanoplexes significantly silenced gene expression for galectin-1 and reversed the effects of methamphetamine on galectin-1 gene expression. Moreover, the effects of methamphetamine on HIV-1 infection were attenuated in the presence of the nanoplex in MDM.||URI:||https://hdl.handle.net/10356/97945
|ISSN:||1557-1890||DOI:||10.1007/s11481-012-9379-7||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||EEE Journal Articles|
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