dc.contributor.authorBarrett, Maria
dc.contributor.authorJahangir, Mohammad M. R.
dc.contributor.authorLee, Changsoo
dc.contributor.authorSmith, Cindy J.
dc.contributor.authorBhreathnach, Niamh
dc.contributor.authorCollins, Gavin
dc.contributor.authorRichards, Karl G.
dc.contributor.authorO’Flaherty, Vincent
dc.date.accessioned2013-10-31T01:41:11Z
dc.date.available2013-10-31T01:41:11Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationBarrett, M., Jahangir, M. M. R., Lee, C., Smith, C. J., Bhreathnach, N., Collins, G., et al. (2013). Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites. Environmental science and pollution research, 20(9), 6646-6657.en_US
dc.identifier.issn0944-1344en_US
dc.identifier.urihttp://hdl.handle.net/10220/17095
dc.description.abstractThis study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater zones: (1) subsoil, (2) subsoil to bedrock interface, and (3) bedrock. The gene copy concentrations (GCCs), as gene copies per liter, for bacterial 16S rRNA genes and the denitrifying functional genes, nirK, nirS, and nosZ, were determined using quantitative polymerase chain reaction assays. The results were related to gaseous nitrogen emissions and to the physicochemical properties of the four sites. Overall, nirK and nirS abundance appeared to show no significant correlation to N2O production (P = 0.9989; P = 0.3188); and no significant correlation was observed between nosZ and excess N2 concentrations (P = 0.0793). In the majority of piezometers investigated, the variation of nirK and nirS gene copy concentrations was considered significant (P < 0.0001). Dissolved organic carbon (DOC) decreased with aquifer depth and ranged from 1.0–4.0 mg l−1, 0.9–2.4 mg l−1, and 0.8–2.4 mg l−1 within piezometers located in the subsoil, subsoil/bedrock interface, and bedrock depths, respectively. The availability of increasing DOC and the depth of the water table were positively correlated with increasing nir and nosZ GCCs (P = 0.0012). A significant temporal correlation was noted between nirS and piezometer depth (P < 0.001). Interestingly, the nirK, nirS, and nosZ GCCs varied between piezometer depths within specific sites, while GCCs remained relatively constant from site to site, thus indicating no direct impact of agricultural land management strategies investigated on denitrifier abundance.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesEnvironmental science and pollution researchen_US
dc.subjectDRNTU::Engineering::Environmental engineering::Environmental pollution
dc.titleAbundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sitesen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s11356-013-1729-3


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