dc.contributor.authorQian, Chen
dc.contributor.authorWong, Chui Ching
dc.contributor.authorSwarup, Sanjay
dc.contributor.authorChiam, Keng-Hwee
dc.date.accessioned2013-11-07T07:28:43Z
dc.date.available2013-11-07T07:28:43Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationQian, C., Wong, C. C., Swarup, S., & Chiam, K. H. (2013). Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility. Applied and environmental microbiology, 79(15), 4734-4743.en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttp://hdl.handle.net/10220/17396
dc.description.abstractWe have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of the cell's rotational trajectories. The second applies piecewise linear approximation to the accumulated rotation curve to reduce noise and separate the motion of bacteria into phases. Thus, it can separate counterclockwise (CCW) and clockwise (CW) rotations distinctly and measure rotational speed accurately. Using this program, we analyzed the properties of tethered Pseudomonas aeruginosa and Pseudomonas putida cells for the first time. We found that the Pseudomonas flagellar motor spends equal time in both CCW and CW phases and that it rotates with the same speed in both phases. In addition, we discovered that the cell body can remain stationary for short periods of time, leading to the existence of a third phase of the flagellar motor which we call “pause.” In addition, P. aeruginosa cells adopt longer run lengths, fewer pause frequencies, and shorter pause durations as part of their chemotactic response. We propose that one purpose of the pause phase is to allow the cells to turn at a large angle, where we show that pause durations in free-swimming cells positively correlate with turn angle sizes. Taken together, our results suggest a new “run-reverse-turn” paradigm for polar-flagellated Pseudomonas motility that is different from the “run-and-tumble” paradigm established for peritrichous Escherichia coli.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesApplied and environmental microbiologyen_US
dc.subjectDRNTU::Science::Biological sciences::Microbiology
dc.titleBacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motilityen_US
dc.typeJournal Article
dc.identifier.doihttp://dx.doi.org/10.1128/AEM.01027-13


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record