dc.contributor.authorLoo, Adeline Huiling
dc.contributor.authorBonanni, Alessandra
dc.contributor.authorPumera, Martin
dc.date.accessioned2013-11-08T06:40:58Z
dc.date.available2013-11-08T06:40:58Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationLoo, A. H., Bonanni, A., & Pumera, M. (2013). Biorecognition on graphene : physical, covalent, and affinity immobilization methods exhibiting dramatic differences. Chemistry - an Asian journal, 8(1), 198-203.en_US
dc.identifier.issn1861-4728en_US
dc.identifier.urihttp://hdl.handle.net/10220/17492
dc.description.abstractThe preparation of biorecognition layers on the surface of a sensing platform is a very crucial step for the development of sensitive and selective biosensors. Different protocols have been used thus far for the immobilization of biomolecules onto various electrode surfaces. In this work, we investigate how the protocol followed for the immobilization of a DNA aptamer affects the performance of the fabricated thrombin aptasensor. Specifically, the differences in selectivity and optimum amount of immobilized aptamer of the fabricated aptasensors adopting either physical, covalent, or affinity immobilization were compared. It was discovered that while all three methods of immobilization uniformly show a similar optimum amount of immobilized aptamer, physical, and covalent immobilization methods exhibit higher selectivity than affinity immobilization. Hence, it is believed that our findings are very important in order to optimize and improve the performance of graphene-based aptasensors.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesChemistry - an Asian journalen_US
dc.subjectDRNTU::Science::Chemistry
dc.titleBiorecognition on graphene : physical, covalent, and affinity immobilization methods exhibiting dramatic differencesen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1002/asia.201200756


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