Towards an optimal and unbiased approach for tumor cell isolation
Sajay, Bhuvanendran Nair Gourikutty
Chung, Wong Chee
Puiu, Poenar Daniel
Rahman, Abdur Rub Abdur
Date of Issue2013
School of Electrical and Electronic Engineering
Our current understanding of clinical significance or the lack thereof of circulating tumor cells (CTCs) is biased by the technology used to isolate these rare cells. Despite the presence of a vast number of academic and commercial technologies, the lack of a standardized and optimized platform has been widely noted. We present a negative enrichment approach, integrating WBC depletion and chemical-free RBC depletion in the same setup without the need for centrifugation, washing or multiple sample handling steps. This approach achieves an average of >90 % recovery of spiked tumor cells and >99 % total WBC depletion in whole blood across multiple cell lines, in a simple and easy-to-use assay. The results presented herein and ongoing improvements aim to fulfill the need for a highly reliable, unbiased, standardized, and optimized CTC isolation platform, using component technologies that are validated for cell isolation.
DRNTU::Engineering::Electrical and electronic engineering
© 2013 Springer Science+Business Media New York. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomedical Microdevices, Springer Science+Business Media New York. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10544-013-9757-9].