Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/101403
Title: Community structure and functional gene profile of bacteria on healthy and diseased Thalli of the red seaweed Delisea pulchra
Authors: Fernandes, Neil
Steinberg, Peter
Rusch, Doug
Kjelleberg, Staffan
Thomas, Torsten
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Fernandes, N., Steinberg, P., Rusch, D., Kjelleberg, S., & Thomas, T. (2012). Community structure and functional gene profile of bacteria on healthy and diseased Thalli of the red seaweed Delisea pulchra. PLoS ONE, 7(12), e50854.
Series/Report no.: PLoS ONE
Abstract: Disease is increasingly viewed as a major factor in the ecology of marine communities and its impact appears to be increasing with environmental change, such as global warming. The temperate macroalga Delisea pulchra bleaches in Southeast Australia during warm summer periods, a phenomenon which previous studies have indicated is caused by a temperature induced bacterial disease. In order to better understand the ecology of this disease, the bacterial communities associated with threes type of samples was investigated using 16S rRNA gene and environmental shotgun sequencing: 1) unbleached (healthy) D. pulchra 2) bleached parts of D. pulchra and 3) apparently healthy tissue adjacent to bleached regions. Phylogenetic differences between healthy and bleached communities mainly reflected relative changes in the taxa Colwelliaceae, Rhodobacteraceae, Thalassomonas and Parvularcula. Comparative metagenomics showed clear difference in the communities of healthy and diseased D. pulchra as reflected by changes in functions associated with transcriptional regulation, cation/multidrug efflux and non-ribosomal peptide synthesis. Importantly, the phylogenetic and functional composition of apparently healthy tissue adjacent to bleached sections of the thalli indicated that changes in the microbial communities already occur in the absence of visible tissue damage. This shift in unbleached sections might be due to the decrease in furanones, algal metabolites which are antagonists of bacterial quorum sensing. This study reveals the complex shift in the community composition associated with bleaching of Delisea pulchra and together with previous studies is consistent with a model in which elevated temperatures reduce levels of chemical defenses in stressed thalli, leading to colonization or proliferation by opportunistic pathogens or scavengers.
URI: https://hdl.handle.net/10356/101403
http://hdl.handle.net/10220/18685
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0050854
Rights: © 2012 The Authors. This paper was published in PLOS ONE and is made available as an electronic reprint (preprint) with permission of The Authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1371/journal.pone.0050854].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.