Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/101613
Title: Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program
Authors: Vij, Shubha
Eitel, Michael
Ho, Hao Kee
Babu, Deepak
Narasimhan, Vijayashankaranarayanan
Tiku, Varnesh
Westbrook, Jody
Schierwater, Bernd
Roy, Sudipto
Rink, Jochen C.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Vij, S., Rink, J. C., Ho, H. K., Babu, D., Eitel, M., Narasimhan, V., et al. (2012). Evolutionarily ancient association of the FoxJ1 transcription factor with the motile ciliogenic program. PLoS genetics, 8(11), e1003019.
Series/Report no.: PLoS genetics
Abstract: It is generally believed that the last eukaryotic common ancestor (LECA) was a unicellular organism with motile cilia. In the vertebrates, the winged-helix transcription factor FoxJ1 functions as the master regulator of motile cilia biogenesis. Despite the antiquity of cilia, their highly conserved structure, and their mechanism of motility, the evolution of the transcriptional program controlling ciliogenesis has remained incompletely understood. In particular, it is presently not known how the generation of motile cilia is programmed outside of the vertebrates, and whether and to what extent the FoxJ1-dependent regulation is conserved. We have performed a survey of numerous eukaryotic genomes and discovered that genes homologous to foxJ1 are restricted only to organisms belonging to the unikont lineage. Using a mis-expression assay, we then obtained evidence of a conserved ability of FoxJ1 proteins from a number of diverse phyletic groups to activate the expression of a host of motile ciliary genes in zebrafish embryos. Conversely, we found that inactivation of a foxJ1 gene in Schmidtea mediterranea, a platyhelminth (flatworm) that utilizes motile cilia for locomotion, led to a profound disruption in the differentiation of motile cilia. Together, all of these findings provide the first evolutionary perspective into the transcriptional control of motile ciliogenesis and allow us to propose a conserved FoxJ1-regulated mechanism for motile cilia biogenesis back to the origin of the metazoans.
URI: https://hdl.handle.net/10356/101613
http://hdl.handle.net/10220/18689
ISSN: 1553-7404
DOI: http://dx.doi.org/10.1371/journal.pgen.1003019
Rights: © 2012 The Authors. This paper was published in PLOS Genetics and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1371/journal.pgen.1003019].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.