Detection of Listeria monocytogenes by using the polymerase chain reaction
Bessesen, Mary T.
Rotbart, Harley A.
Blaser, Martin J.
Ellison III, Richard T.
Date of Issue1990
School of Chemical and Biomedical Engineering
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
DRNTU::Engineering::Chemical engineering::Chemical processes
Applied and environmental microbiology
© 1990 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Applied And Environmental Microbiology, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://aem.asm.org/content/56/9/2930.full.pdf+html].