dc.contributor.authorDatta, Arnab
dc.contributor.authorQian, Jingru
dc.contributor.authorChong, Ruifen
dc.contributor.authorKalaria, Raj N.
dc.contributor.authorFrancis, Paul
dc.contributor.authorLai, Mitchell K. P.
dc.contributor.authorChen, Christopher P.
dc.contributor.authorSze, Siu Kwan
dc.identifier.citationDatta, A., Qian, J., Chong, R., Kalaria, R. N., Francis, P., Lai, M. K. P., et. al. (2014) Novel Pathophysiological Markers are Revealed by iTRAQ-based Quantitative Clinical Proteomics Approach in Vascular Dementia. Journal of Proteomics.en_US
dc.description.abstractVascular dementia (VaD) is a leading cause of dementia in the elderly together with Alzheimer's disease with limited treatment options. Poor understanding of the pathophysiology underlying VaD is hindering the development of new therapies. Hence, to unravel its underlying molecular pathology, an iTRAQ-2D-LC–MS/MS strategy was used for quantitative analysis of pooled lysates from Brodmann area 21 of pathologically confirmed cases of VaD and matched non-neurological controls. A total of 144 differentially perturbed proteins out of 2284 confidently identified proteins (false discovery rate = 0.3%) were shortlisted for bioinformatics analysis. Western blot analysis of selected proteins using samples from individual patients (n = 10 per group) showed statistically significant increases in the abundance of SOD1 and NCAM and reduced ATP5A in VaD. This suggested a state of hypometabolism and vascular insufficiency along with an inflammatory condition during VaD. Elevation of SOD1 and increasing trend for iron-storage proteins (FTL, FTH1) may be indicative of an oxidative imbalance that is accompanied by an aberrant iron metabolism. The synaptic proteins did not exhibit a generalized decrease in abundance (e.g. syntaxin) in the VaD subjects. This reported proteome offers a reference data set for future basic or translational studies on VaD.en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)
dc.relation.ispartofseriesJournal of proteomicsen_US
dc.rights© 2014 Elsevier B.V. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Proteomics, Elsevier B.V. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.jprot.2014.01.011].en_US
dc.subjectDRNTU::Science::Biological sciences
dc.titleNovel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementiaen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionAccepted versionen_US

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