Fast depth-sensitive fluorescence measurements in turbid media using cone shell configuration
Ong, Yi Hong
Date of Issue2013
School of Chemical and Biomedical Engineering
Depth-sensitive fluorescence spectroscopy in a two-layered epithelial tissue phantom was demonstrated using a cone shell configuration implemented by an axicon lens and a fiber assembly including five rings of collection fibers. Each collection fiber ring was located at a different radial distance away from the center, for which fluorescence measurements from all rings showed a larger range of sensitivities to the top and bottom layers compared to the previously reported cone shell configuration. Furthermore, multiple fluorescence spectra corresponding to a range of successive targeted depths can be obtained simultaneously, which shortened data acquisition dramatically and eliminated the mechanical moving components required for depth-sensitive optical measurements in the previous setup. This new setup therefore would be preferred in a clinical setting.
Journal of biomedical optics
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-Optical Instrumentation Engineers (SPIE). The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/1.JBO.18.11.110503]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.