Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos
Ong, Kimberly J.
Ede, James D.
Stafford, James L.
Ng, Kee Woei
Goss, Greg G.
Loo, Joachim Say Chye
Date of Issue2013
School of Materials Science and Engineering
The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 μg mL−1. However, 30 μg mL−1 needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 μg mL−1 needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 μg mL−1) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.