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Title: In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
Authors: Low, Ying Xiu
De Hoog, Hans-Peter M.
May, Sylvia
Andreasson-Ochsner, Mirjam
Fu, Zhikang
Tan, Darren
Ritz, Sandra
Nallani, Madhavan
Sinner, Eva-Kathrin
Keywords: DRNTU::Engineering::Materials::Biomaterials
Issue Date: 2013
Source: May, S., Andreasson-Ochsner, M., Fu, Z., Low , Y. X., Tan, D., de Hoog, H.-P. M., et al. (2013). In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies. Angewandte Chemie International Edition, 52(2), 749-753.
Series/Report no.: Angewandte chemie international edition
Abstract: The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.
ISSN: 1433-7851
Rights: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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