dc.contributor.authorYildiz, Umit Hakan
dc.contributor.authorDe Hoog, Hans-Peter M.
dc.contributor.authorFu, Zhikang
dc.contributor.authorTomczak, Nikodem
dc.contributor.authorParikh, Atul N.
dc.contributor.authorNallani, Madhavan
dc.contributor.authorLiedberg, Bo
dc.date.accessioned2014-04-07T02:41:02Z
dc.date.available2014-04-07T02:41:02Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationYildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447.en_US
dc.identifier.issn1613-6810en_US
dc.identifier.urihttp://hdl.handle.net/10220/19139
dc.description.abstractSubmicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesSmallen_US
dc.rights© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.subjectDRNTU::Engineering::Materials
dc.titleThird-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartmentsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1002/smll.201300060


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