dc.contributor.authorSetyawati, Magdiel Inggrid
dc.contributor.authorKhoo, Pheng Kian Stella
dc.contributor.authorEng, Bao Hui
dc.contributor.authorXiong, Sijing
dc.contributor.authorZhao, Xinxin
dc.contributor.authorDas, Gautom Kumar
dc.contributor.authorTan, Timothy Thatt Yang
dc.contributor.authorLoo, Joachim Say Chye
dc.contributor.authorLeong, David Tai
dc.contributor.authorNg, Kee Woei
dc.date.accessioned2014-04-09T06:11:57Z
dc.date.available2014-04-09T06:11:57Z
dc.date.copyright2012en_US
dc.date.issued2012
dc.identifier.citationSetyawati, M. I., Khoo, P. K. S., Eng, B. H., Xiong, S., Zhao, X., Das, G. K., et al. (2013). Cytotoxic and genotoxic characterization of titanium dioxide, gadolinium oxide, and poly(lactic-co-glycolic acid) nanoparticles in human fibroblasts. Journal of Biomedical Materials Research Part A, 101A(3), 633-640.en_US
dc.identifier.issn1549-3296en_US
dc.identifier.urihttp://hdl.handle.net/10220/19191
dc.description.abstractEngineered nanomaterials have become prevalent in our everyday life. While the popularity of using nanomaterials in consumer products continues to rise, increasing awareness of nanotoxicology has also fuelled efforts to accelerate our understanding of the ill effects that different nanomaterials can bring to biological systems. In this study, we investigated the potential cytotoxicity and genotoxicity of three nanoparticles: titanium dioxide (TiO2), terbium-doped gadolinium oxide (Tb-Gd2O3), and poly(lactic-co-glycolic acid) (PLGA). To evaluate nanoparticle-induced genotoxicity more realistically, a human skin fibroblast cell line (BJ) with less mutated genotype compared with cancer cell line was used. The nanoparticles were first characterized by size, morphology, and surface charge. Cytotoxicity effects of the nanoparticles were then evaluated by monitoring the proliferation of treated BJ cells. Genotoxic influence was ascertained by profiling DNA damage via detection of γH2AX expression. Our results suggested that both TiO2 and Tb-Gd2O3 nanoparticles induced cytotoxicity in a dose dependent way on BJ cells. These two nanomaterials also promoted genotoxicity via DNA damage. On the contrary, PLGA nanoparticles did not induce significant cytotoxic or genotoxic effects on BJ cells.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of biomedical materials research part Aen_US
dc.rights© 2012 Wiley Periodicals, Inc.en_US
dc.subjectDRNTU::Engineering::Materials::Nanostructured materials
dc.titleCytotoxic and genotoxic characterization of titanium dioxide, gadolinium oxide, and poly(lactic-co-glycolic acid) nanoparticles in human fibroblastsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.a.34363


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