Next-generation transgenic mice for optogenetic analysis of neural circuits
Augustine, George J.
Lee, Li Ming
Lo, Shun Qiang
Ting, Jonathan T.
Date of Issue2013
Lee Kong Chian School of Medicine
Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
Frontiers in neural circuits
© The Author(s). This paper was published in Frontiers in Neural Circuits and is made available as an electronic reprint (preprint) with permission of Frontiers. The paper can be found at the following official DOI: http://dx.doi.org/10.3389/fncir.2013.00160. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.