Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
Tan, Amy Giin-Yu
Tan, Swee Ngin
Date of Issue2013
School of Civil and Environmental Engineering
Nanyang Environment and Water Research Institute
A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R2 ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers.
Journal of Bioscience and Bioengineering
© 2013 The Society for Biotechnology, Japan. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Bioscience and Bioengineering, The Society for Biotechnology, Japan. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1016/j.jbiosc.2013.08.020].