Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104049
Title: Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients
Authors: Kaur, Palvinder
Khong, Wei Xin
Wee, Sue Yuen
Tan, Eng Lee
Pipper, Juergen
Koay, Evelyn
Ng, Kah Ying
Yap, Joe Kwan
Chew, Kuan Kiat
Tan, Mei Ting
Leo, Yee Sin
Inoue, Masafumi
Ng, Oon Tek
Keywords: DRNTU::Science::Medicine
Issue Date: 2014
Source: Kaur, P., Khong, W. X., Wee, S. Y., Tan, E. L., Pipper, J., Koay, E., et al. (2014). Clinical Evaluation of a Low Cost, In-House Developed Real-Time RT-PCR Human Immunodeficiency Virus Type 1 (HIV-1) Quantitation Assay for HIV-1 Infected Patients. PLoS ONE, 9(3).
Series/Report no.: PLoS ONE
Abstract: Objectives HIV-1 viral quantitation is essential for treatment monitoring. An in-house assay would decrease financial barriers to access. Materials and Methods A real-time competitive RT-PCR in house assay (Sing-IH) was developed in Singapore. Using HXB2 as reference, the assay's primers and probes were designed to generate a 183-bp product that overlaps a portion of the LTR region and gag region. A competitive internal control (IC) was included in each assay to monitor false negative results due to inhibition or human error. Clinical evaluation was performed on 249 HIV-1 positive patient samples in comparison with the commercially available Generic HIV Viral Load assay. Correlation and agreement of results were assessed for plasma HIV-1 quantification with both assays. Results The assay has a lower limit of detection equivalent to 126 copies/mL of HIV-1 RNA and a linear range of detection from 100–1000000 copies/mL. Comparative analysis with reference to the Generic assay demonstrated good agreement between both assays with a mean difference of 0.22 log10 copies/mL and 98.8% of values within 1 log10 copies/mL range. Furthermore, the Sing-IH assay can quantify HIV-1 group M subtypes A–H and group N isolates adequately, making it highly suitable for our region, where subtype B and CRF01_AE predominate. Conclusions With a significantly lower running cost compared to commercially available assays, the broadly sensitive Sing-IH assay could help to overcome the cost barriers and serve as a useful addition to the currently limited HIV viral load assay options for resource-limited settings.
URI: https://hdl.handle.net/10356/104049
http://hdl.handle.net/10220/19482
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0089826
Rights: © 2014 Kaur et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.