Comparison of cone and cone shell configuration for depth sensitive fluorescence measurements in turbid media
Ong, Yi Hong
Date of Issue2014
Photonic Therapeutics and Diagnostics X
School of Chemical and Biomedical Engineering
We have developed a novel cone shell illumination and detection configurations using combination of axicon lenses for depth sensitive fluorescence spectroscopy. The probe was demonstrated experimentally to be able to selectively detecting fluorescence from different depths from a two-layered turbid agar phantom. In addition to enhanced contrast of subsurface fluorescence measurement as compared to a conventional cone configuration implemented by a microscope objective lens, the axicon lenses based setup eliminated the need of moving the objective lens up or down to achieve depth sensitive measurements, which effectively improves the consistency of optical coupling thus would be preferred in a clinical setting.
© 2014 SPIE. This paper was published in Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X and is made available as an electronic reprint (preprint) with permission of SPIE. The paper can be found at the following official DOI: http://dx.doi.org/10.1117/12.2038834. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.