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      Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses

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      Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses.pdf (3.518Mb)
      Author
      Kim, Jinsook
      Lee, Soojung
      Tsuda, Sachiko
      Zhang, Xuying
      Asrican, Brent
      Gloss, Bernd
      Feng, Guoping
      Augustine, George J.
      Date of Issue
      2014
      School
      Lee Kong Chian School of Medicine
      Research Centre
      Research Techno Plaza
      Version
      Published version
      Abstract
      We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions.
      Subject
      DRNTU::Science::Medicine
      Type
      Journal Article
      Series/Journal Title
      Cell reports
      Rights
      This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). © 2014 The Authors. Published by Elsevier Inc. User rights governed by an Open Access license.
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      • LKCMedicine Journal Articles
      http://dx.doi.org/10.1016/j.celrep.2014.04.047
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