Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/101569
Title: The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis
Authors: Gindin, Galina
Mendel, Zvi
Levitin, Bella
Kumar, Pradeep
Kuznetsova, Tatiana
Levi, Tal
Shahi, Preeti
Khasdan, Vadim
Weinthal, Dan
Einav, Monica
Kushmaro, Ariel
Protasov, Alex
Zaritsky, Arieh
Ben-Dov, Eitan
Keywords: DRNTU::Science::Biological sciences::Botany
DRNTU::Engineering::Materials
Issue Date: 2013
Source: Gindin, G., Mendel, Z., Levitin, B., Kumar, P., Levi, T., Shahi, P., et al. (2013). The basis for rootstock resilient to Capnodis species: screening for genes encoding δ -endotoxins from Bacillus thuringiensis . Pest Management Science, 70(8), 1283–1290.
Series/Report no.: Pest management science
Abstract: BACKGROUND Conventional methods often fail to control the flatheaded borers Capnodis spp., major pests of stone fruit trees; the larvae are protected from insecticides and predation because they feed deep in the roots. A potential solution is transgenic trees producing in their roots toxic compounds such as Cry proteins of Bacillus thuringiensis (Bt). RESULTS Toxicities against Capnodis larvae were demonstrated by exploiting a recently designed artificial larval diet and an available collection of field isolated Bt. An isolate of Bt tenebrionis (Btt) from commercial bioinsecticide (Novodor) displayed LC50 and LC95 values of 3.2 and 164 mg g−1, respectively, against neonates of Capnodis tenebrionis, whereas values of the most toxic field isolate K-7 were 1.9 and 25.6 mg g−1 respectively. Weights of surviving larvae after 1 month on diets containing low concentrations of K-7 (0.1–1.0 mg g−1) were lower than on Btt or untreated larvae. K-7 was also toxic against larvae of C. cariosa and C. miliaris and found to harbour genes encoding Cry9Ea-like and Cry23Aa/Cry37Aa binary toxins. CONCLUSION Larvae of Capnodis spp. are susceptible to Bt Cry toxins. Expressing cry genes active against these pests thus seems a feasible solution towards production of transgenic rootstock trees resilient to the pest.
URI: https://hdl.handle.net/10356/101569
http://hdl.handle.net/10220/19728
ISSN: 1526-498X
DOI: http://dx.doi.org/10.1002/ps.3694
Rights: © 2013 Society of Chemical Industry.
metadata.item.grantfulltext: none
metadata.item.fulltext: No Fulltext
Appears in Collections:MSE Journal Articles

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