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Title: Cell depletion in mice that express diphtheria toxin receptor under the control of SiglecH encompasses more than plasmacytoid dendritic cells
Authors: Swiecki, Melissa
Wang, Yaming
Riboldi, Elena
Kim, Alfred H. J.
Dzutsev, Amiran
Gilfillan, Susan
Vermi, William
Ruedl, Christiane
Trinchieri, Giorgio
Colonna, Marco
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2014
Source: Swiecki, M., Wang, Y., Riboldi, E., Kim, A. H. J., Dzutsev, A., Gilfillan, S., et al. (2014). Cell Depletion in Mice That Express Diphtheria Toxin Receptor under the Control of SiglecH Encompasses More Than Plasmacytoid Dendritic Cells. The Journal of Immunology, 192(9), 4409-4416.
Series/Report no.: The journal of immunology
Abstract: Plasmacytoid dendritic cells (pDC) produce IFN-I in response to viruses and are routinely identified in mice by SiglecH expression. SiglecH is a sialic acid–binding Ig-like lectin that has an immunomodulatory role during viral infections. In this study, we evaluated the impact of SiglecH deficiency on cytokine responses in the presence and absence of pDC. We found that lack of SiglecH enhanced IFN-I responses to viral infection, regardless of whether pDC were depleted. We also examined the expression pattern of SiglecH and observed that it was expressed by specialized macrophages and progenitors of classical dendritic cells and pDC. Accordingly, marginal zone macrophages and pDC precursors were eliminated in newly generated SiglecH–diphtheria toxin receptor (DTR)–transgenic (Tg) mice but not in CLEC4C-DTR–Tg mice after diphtheria toxin (DT) treatment. Using two bacterial models, we found that SiglecH-DTR–Tg mice injected with DT had altered bacterial uptake and were more susceptible to lethal Listeria monocytogenes infection than were DT-treated CLEC4C-DTR–Tg mice. Taken together, our findings suggest that lack of SiglecH may affect cytokine responses by cell types other than pDC during viral infections, perhaps by altering viral distribution or burden, and that cell depletion in SiglecH-DTR–Tg mice encompasses more than pDC.
ISSN: 0022-1767
Rights: © 2014 American Association of Immunologists.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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