View Item 
      •   Home
      • 1. Schools
      • College of Engineering
      • School of Chemical and Biomedical Engineering (SCBE)
      • SCBE Journal Articles
      • View Item
      •   Home
      • 1. Schools
      • College of Engineering
      • School of Chemical and Biomedical Engineering (SCBE)
      • SCBE Journal Articles
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      Subject Lookup

      Browse

      All of DR-NTUCommunities & CollectionsTitlesAuthorsBy DateSubjectsThis CollectionTitlesAuthorsBy DateSubjects

      My Account

      Login

      Statistics

      Most Popular ItemsStatistics by CountryMost Popular Authors

      About DR-NTU

      Role of HBV replication in host cell metabolism : a proteomics analysis

      Thumbnail
      Author
      Sadrolodabaee, Laleh
      Low, Tiong Keng
      Feng, Huixing
      Chen, Wei Ning
      Date of Issue
      2013
      School
      School of Chemical and Biomedical Engineering
      Abstract
      Hepatocellular carcinoma (HCC) with more than 700,000 deaths every year is the most prevalent type of liver cancer and a global concern. It is the fifth most common cancer worldwide and has a poor general prognosis. Chronic hepatitis B virus (HBV) infection is a major cause of HCC. The HBV-infected individual has 100 times higher risk of developing HCC. The x protein of HBV (HBx) has been shown to involve in the development of HCC. In this study, the association between HBV replication and the host cell metabolism is investigated. HepG2 cells are transfected with different genotypes of HBx and total proteins are extracted and analyzed using LC-MS/MS. Our proteomics results indicates that a number of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PK), Phosphoglyceratekinase (PGK) and Lactate dehydrogenase (LDH) are significantly up-regulated in HepG2 cells transfected by HBx comparing with control group. These findings suggest that HBV replication could alter host cell metabolism by increasing the rate of glycolysis to provide important metabolic requirements for nucleotides, amino acids and lipids synthesis. Hence, our proteomics approach may provide candidate biomarkers to improve the diagnosis of HBVrelated HCC patients.
      Subject
      DRNTU::Engineering::Bioengineering
      Type
      Journal Article
      Series/Journal Title
      Current proteomics
      Rights
      © 2013 Bentham Science Publishers.
      Collections
      • SCBE Journal Articles
      http://dx.doi.org/10.2174/15701646112099990008
      Get published version (via Digital Object Identifier)

      Show full item record


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       

      DCSIMG