Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103645
Title: A new molecular surveillance system for Leishmaniasis
Authors: Pandey, Kishor
Pandey, Basu Dev
Mallik, Arun Kumar
Acharya, Jyoti
Kato, Kentaro
Kaneko, Osamu
Ferreira, Pedro Eduardo
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2014
Source: Pandey, K., Pandey, B. D., Mallik, A. K., Acharya, J., Kato, K., Kaneko, O., et al. (2014). A New Molecular Surveillance System for Leishmaniasis. American Journal of Tropical Medicine and Hygiene, 13-0329.
Series/Report no.: American journal of tropical medicine and hygiene
Abstract: Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.
URI: https://hdl.handle.net/10356/103645
http://hdl.handle.net/10220/20298
ISSN: 0002-9637
DOI: http://dx.doi.org/10.4269/ajtmh.13-0329
Rights: © 2014 American Society of Tropical Medicine and Hygiene. This paper was published in American Journal of Tropical Medicine and Hygiene and is made available as an electronic reprint (preprint) with permission of American Society of Tropical Medicine and Hygiene. The paper can be found at the following official DOI: [http://dx.doi.org/10.4269/ajtmh.13-0329]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
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