A new molecular surveillance system for Leishmaniasis
Pandey, Basu Dev
Mallik, Arun Kumar
Ferreira, Pedro Eduardo
Date of Issue2014
School of Biological Sciences
Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.
American journal of tropical medicine and hygiene
© 2014 American Society of Tropical Medicine and Hygiene. This paper was published in American Journal of Tropical Medicine and Hygiene and is made available as an electronic reprint (preprint) with permission of American Society of Tropical Medicine and Hygiene. The paper can be found at the following official DOI: [http://dx.doi.org/10.4269/ajtmh.13-0329]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.