Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104966
Title: TSpred : a web server for the rational design of temperature-sensitive mutants
Authors: Tan, Kuan Pern
Khare, Shruti
Varadarajan, Raghavan
Madhusudhan, Mallur Srivatsan
Keywords: DRNTU::Engineering::Computer science and engineering
Issue Date: 2014
Source: Tan, K. P., Khare, S., Varadarajan, R., & Madhusudhan, M. S. (2014). TSpred: a web server for the rational design of temperature-sensitive mutants. Nucleic Acids Research, 42(W1), W277-W284.
Series/Report no.: Nucleic acids research
Abstract: Temperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by random mutagenesis and then selected though laborious large-scale screening. Our web server, TSpred (http://mspc.bii.a-star.edu.sg/TSpred/), now enables users to rationally design Ts mutants for their proteins of interest. TSpred uses hydrophobicity and hydrophobic moment, deduced from primary sequence and residue depth, inferred from 3D structures to predict/identify buried hydrophobic residues. Mutating these residues leads to the creation of Ts mutants. Our method has been experimentally validated in 36 positions in six different proteins. It is an attractive proposition for Ts mutant engineering as it proposes a small number of mutations and with high precision. The accompanying web server is simple and intuitive to use and can handle proteins and protein complexes of different sizes.
URI: https://hdl.handle.net/10356/104966
http://hdl.handle.net/10220/20390
ISSN: 0305-1048
DOI: 10.1093/nar/gku319
Schools: School of Computer Engineering 
Rights: © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCSE Journal Articles

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