dc.contributor.authorTan, Kuan Pern
dc.contributor.authorKhare, Shruti
dc.contributor.authorVaradarajan, Raghavan
dc.contributor.authorMadhusudhan, Mallur Srivatsan
dc.date.accessioned2014-08-25T02:12:36Z
dc.date.available2014-08-25T02:12:36Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.citationTan, K. P., Khare, S., Varadarajan, R., & Madhusudhan, M. S. (2014). TSpred: a web server for the rational design of temperature-sensitive mutants. Nucleic Acids Research, 42(W1), W277-W284.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10220/20390
dc.description.abstractTemperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by random mutagenesis and then selected though laborious large-scale screening. Our web server, TSpred (http://mspc.bii.a-star.edu.sg/TSpred/), now enables users to rationally design Ts mutants for their proteins of interest. TSpred uses hydrophobicity and hydrophobic moment, deduced from primary sequence and residue depth, inferred from 3D structures to predict/identify buried hydrophobic residues. Mutating these residues leads to the creation of Ts mutants. Our method has been experimentally validated in 36 positions in six different proteins. It is an attractive proposition for Ts mutant engineering as it proposes a small number of mutations and with high precision. The accompanying web server is simple and intuitive to use and can handle proteins and protein complexes of different sizes.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesNucleic acids researchen_US
dc.rights© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectDRNTU::Engineering::Computer science and engineering
dc.titleTSpred : a web server for the rational design of temperature-sensitive mutantsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Computer Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gku319
dc.description.versionPublished versionen_US


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