In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation
Ferhan, Abdul Rahim
Date of Issue2014
School of Chemical and Biomedical Engineering
We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.
© 2014 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by RSC Advances, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/c3ra47012a].