dc.contributor.authorSun, Yi
dc.contributor.authorSatyanarayan, M.V.D.
dc.contributor.authorNguyen, Nam Trung
dc.contributor.authorKwok, Yien Chian
dc.identifier.citationSun, Y., Satyanarayan, M., Nguyen, N. T., & Kwok, Y. C. (2008). Continuous flow polymerase chain reaction using a hybrid PMMA-PC microchip with improved heat tolerance. Sensors and actuators B: chemical, 130(2), 836-841.en_US
dc.description.abstractRecently, polymeric materials have been explored as more versatile alternatives for the fabrication of polymerase chain reaction (PCR) microchips. Poly(methyl methacrylate) (PMMA) is a popular substrate material due to its high mechanical stability, good chemical properties and most importantly, its suitability for cheap and simple CO2 laser ablation. However, it has a low glass transition temperature (Tg) of 105 °C, which is just above the denaturation temperature for PCR, thus the bond integrity is compromised. Polycarbonate (PC) is preferred as a substrate for PCR microchip as it has a higher Tg of 150 °C; but since its thermal properties are not suitable for CO2 laser light, the more expensive excimer laser has to be employed. Here we report a novel hybrid PMMA-PC microchip by bonding a PC cover plate with a PMMA substrate containing microchannel which is fabricated by CO2 laser ablation. This hybrid microchip has improved heat tolerance, such that the bonding integrity is sustained at the denaturation temperature. DNA amplification is found to be more efficiently performed in a PMMA-PC microchip than in a PMMA-PMMA microchip.en_US
dc.format.extent5 p.en_US
dc.relation.ispartofseriesSensors and actuators B: chemicalen_US
dc.rights© 2007 Elsevier B.V.en_US
dc.subjectDRNTU::Engineering::Aeronautical engineering::Materials of construction
dc.titleContinuous flow polymerase chain reaction using a hybrid PMMA-PC microchip with improved heat toleranceen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolNational Institute of Educationen_US

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