Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells
Dinish, U. S.
Kong, Kien Voon
Date of Issue2013
School of Electrical and Electronic Engineering
In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated the receptor-mediated delivery of antibody conjugated gold nanospheres and gold nanorods into EGFR receptor-positive oral squamous cell carcinoma cell line by using dark-field microscopy technique. Our result suggests that gold nanospheres and gold nanorods formulations could provide up to 7 types of plasmon scattering probes based on their tunability range with a 100 nm spectral separation in absorption bands.
Journal of biomedical nanotechnology
© 2013 American Scientific Publishers.