dc.contributor.authorMuthukumar, Subramanian
dc.contributor.authorRajkumar, Ramalingam
dc.contributor.authorRajesh, Durairaj
dc.contributor.authorSaibaba, Ganesan
dc.contributor.authorLiao, Chen-Chung
dc.contributor.authorArchunan, Govindaraju
dc.contributor.authorPadmanabhan, Parasuraman
dc.contributor.authorGulyas, Balazs
dc.date.accessioned2014-12-09T08:25:43Z
dc.date.available2014-12-09T08:25:43Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.citationMuthukumar, S., Rajkumar, R., Rajesh, D., Saibaba, G., Liao, C.-C., Archunan, G., et al. (2014). Exploration of salivary proteins in buffalo : an approach to find marker proteins for estrus. The FASEB journal, 28(11), 4700-4709.en_US
dc.identifier.urihttp://hdl.handle.net/10220/24402
dc.description.abstractSaliva is considered as the best source of biological material for biomarker discovery studies since it is noninvasive in comparison to other body sources. Usually buffalo cannot precisely express estrus signals. Hence, there is a need for concise methods to detect the time of estrus to ensure the success of artificial insemination. Therefore, we have established a reference proteome map on the whole saliva of buffalo during their estrous cycle with special reference to estrus. Nearly 12 bands have been observed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of whole saliva. Collectively, 179 proteins are identified with respect to different phases of the estrous cycle using mass spectrometry. On the whole, 37 proteins are exclusively expressed in the estrus phase, which include β-enolase, Toll-like receptor (TLR) 4, clusterin, lactoperoxidase, serotransferrin, TGM3, UBA6, and transducin. Among the proteins, β-enolase and TLR 4 were validated, and their specific expression was found during estrus as compared to other phases using immunoblot. The functional annotation reveals many as binding proteins in the estrus saliva when compared to the other phases. The present findings conclude that the proteomic approach adopted to identify the proteins from buffalo saliva around the estrous cycle may provide a new tool for screening the estrus phase. The results further conclude that the specific expression of β-enolase and TLR 4 can be taken as the indicator of estrus in buffalo.—Muthukumar, S., Rajkumar, R., Rajesh, D., Saibaba, G., Liao, C.-C., Archunan, G., Padmanabhan, P., Gulyas, B. Exploration of salivary proteins in buffalo: an approach to find marker proteins for estrus.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesThe FASEB journalen_US
dc.rights© 2014 FASEB.en_US
dc.subjectDRNTU::Science::Biological sciences::Zoology
dc.titleExploration of salivary proteins in buffalo : an approach to find marker proteins for estrusen_US
dc.typeJournal Article
dc.contributor.schoolLee Kong Chian School of Medicine
dc.identifier.doihttp://dx.doi.org/10.1096/fj.14-252288


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