Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103191
Title: The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier
Authors: Tran, Cindy S.
Rangel, Stephanie M.
Almblad, Henrik
Kierbel, Arlinet
Givskov, Michael
Tolker-Nielsen, Tim
Hauser, Alan R.
Engel, Joanne N.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2014
Source: Tran, C. S., Rangel, S. M., Almblad, H., Kierbel, A., Givskov, M., Tolker-Nielsen, T., et al. (2014). The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier. PLoS Pathogens, 10(11), e1004479-.
Series/Report no.: PLoS Pathogens
Abstract: Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known about biofilm formation at the epithelial barrier. We have previously shown that when added to the apical surface of polarized epithelial cells, P. aeruginosa rapidly forms cell-associated aggregates within 60 minutes of infection. By confocal microscopy we now show that cell-associated aggregates exhibit key characteristics of biofilms, including the presence of extracellular matrix and increased resistance to antibiotics compared to planktonic bacteria. Using isogenic mutants in the type III secretion system, we found that the translocon, but not the effectors themselves, were required for cell-associated aggregation on the surface of polarized epithelial cells and at early time points in a murine model of acute pneumonia. In contrast, the translocon was not required for aggregation on abiotic surfaces, suggesting a novel function for the type III secretion system during cell-associated aggregation. Supernatants from epithelial cells infected with wild-type bacteria or from cells treated with the pore-forming toxin streptolysin O could rescue aggregate formation in a type III secretion mutant, indicating that cell-associated aggregation requires one or more host cell factors. Our results suggest a previously unappreciated function for the type III translocon in the formation of P. aeruginosa biofilms at the epithelial barrier and demonstrate that biofilms may form at early time points of infection.
URI: https://hdl.handle.net/10356/103191
http://hdl.handle.net/10220/24407
ISSN: 1553-7374
DOI: http://dx.doi.org/10.1371/journal.ppat.1004479
Rights: © 2014 Tran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCELSE Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.