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Title: Molecularly imprinted polymer waveguides for direct optical detection of low-molecular-weight analytes
Authors: Sharma, Nityanand
Petri, Christian
Jonas, Ulrich
Bach, Monika
Tovar, Günter
Mrkvová, Kateřina
Vala, Milan
Homola, Jiří
Knoll, Wolfgang
Dostálek, Jakub
Keywords: DRNTU::Science::Chemistry::Physical chemistry::Molecular structure and bonding
Issue Date: 2014
Source: Sharma, N., Petri, C., Jonas, U., Bach, M., Tovar, G., Mrkvová, K., et al. (2014). Molecularly imprinted polymer waveguides for direct optical detection of low-molecular-weight analytes. Macromolecular chemistry and physics, 215(23), 2295-2304.
Series/Report no.: Macromolecular chemistry and physics
Abstract: New composite layer architecture of 3D hydrogel polymer network that is loaded with mole­cularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low-molecular-weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding-matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label-free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photo-crosslinkable poly(N-isopropylacrylamide)-based hydrogel and poly[(ethylene glycol dimethylacrylate)-(methacrylic acid)] nanoparticles that are imprinted with l-Boc-phenylalanine-anilide (l-BFA, molecular weight 353 g mol−1). Titration experiments with the specific target and other structurally similar reference compounds show good specificity and limit of detection for target l-BFA as low as 2 × 10−6 m.
ISSN: 1022-1352
DOI: 10.1002/macp.201400260
Rights: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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